Showing 11–14 of 14 results

Oxidases

GECCO collection of oxidases comprise various alcohol, amine and carbohydrate oxidases. Main members of this collection are listed below. Flagship oxidase is certainly 5-Hydroxymethylfurfural oxidase (HMFO): this enzyme has been shown to be able to convert HMF into the polymer building block FDCA. The biocatalyst can also be used for other reactions which includes oxidations of thiols.

  1. Alcohol oxidase (AOX) Phanerochaete chrysosporium
  2. Alditol oxidase (HOT-ALDO) Acidothermus cellulolyticus
  3. Alditol oxidase (ALDO) Streptomyces coelicolor A3(2)
  4. Chitooligosaccharide oxidase (CHITO) Fusarium graminearum
  5. Q268R/G270E/S410R (CHITO) Fusarium graminearum
  6. Eugenol oxidase (EUGO) Rhodococcus jostii RHA1
  7. 5-Hydroxymethylfurfural oxidase (HMFO) Methylovorus strain MP688
  8. Isoamyl alcohol oxidase (IAAO) Aspergillus nidulans A4
  9. Vanillyl alcohol oxidase       (VAO) Penicillium simplicissimum
  10. 6-Hydroxy-d-nicotine oxidase Arthrobacter nicotinovorans
  11. Putrescine oxidase (PuO) Rhodococcus erythropolis NCIMB 11540
  12. Putative copper oxidase Thermobifida fusca

P450 Monooxygenases

Cytochrome P450 monooxygenases (P450s) are versatile biocatalysts that catalyze the regio- and stereospecific oxidation of non-activated hydrocarbons under mild conditions, which is a challenging task for chemical catalysts. Over the past decade impressive advances have been achieved via protein engineering with regard to activity, stability and specificity of P450s.

We are offering prototype P450 enzyme, P450 BM3 and several other members of this powerful biocatalysts, together with possibility of delivering specific mutants.

  1. P450 BM3 fused to phosphite dehydrogenase – this bifunctional biocatalyst can perform selective oxidations using phosphite to enable regeneration of NADPH (for more details see Beyer, N., Kulig, J.K., Bartsch, A. et al. Appl Microbiol Biotechnol (2017) 101: 2319. doi:10.1007/s00253-016-7993-7)
  2. P450 from Thermobifida fusca

Penicillin acylases

Page is under construction. We are offering various thermostable and engineered penicillin acylases.

Please make an inquiry.

Transaminases

  1. VfTA (4E3Q) Vibrio fluvialis
  2. CvTA (4BA4) Chromobacterium violaceum
  3. ATA117 Rd11 (3WWJ) Mut.TA from Arthrobacter
  4. VpTA (4AOA, AAO9) Variovorax paradoxus