P450 Monooxygenases

Cytochrome P450 monooxygenases (P450s) are versatile biocatalysts that catalyze the regio- and stereospecific oxidation of non-activated hydrocarbons under mild conditions, which is a challenging task for chemical catalysts. Over the past decade impressive advances have been achieved via protein engineering with regard to activity, stability and specificity of P450s.

We are offering prototype P450 enzyme, P450 BM3 and several other members of this powerful biocatalysts, together with possibility of delivering specific mutants.

1. P450 BM3 fused to phosphite dehydrogenase – this bifunctional biocatalyst can perform selective oxidations using phosphite to enable regeneration of NADPH (for more details see Beyer, N., Kulig, J.K., Bartsch, A. et al. Appl Microbiol Biotechnol (2017) 101: 2319. doi:10.1007/s00253-016-7993-7)
2. P450 from Thermobifida fusca

3. Self-Sufficient Cytochrome P450 from Thermothelomyces thermophila (https://doi.org/10.1002/adsc.201900076)